Loading and correcting TrackMate dataTo start, TrackMate XML data needs to be read into R. It may need recalibrating if the data were captured with incorrect parameters. |
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Read TrackMate XML output files. |
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Correct distance and time of imported TrackMate data. |
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Automated reportsTrackMateR can be used manually to have fine control over your analysis, however if you just want to analyse your data with default parameters, these two functions will process one dataset, or many |
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Report Dataset |
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Compare datasets |
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Analysing track dynamicsTo analyse track dynamics, there are several functions to calculate them. These functions are called (with set parameters) in the automated workflows and all of them must be called to make a report. |
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Calculate alpha (relationship between MSD and normal diffusion) |
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Calculate CVE (covariance-based estimator) |
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Calculate fractal dimension (FD) |
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Calculate Jump Distance (JD) |
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Calculate Mean Squared Displacement (MSD) |
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Calculate density of tracks |
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Fitting jump distance (JD) data |
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Generating reports and summariesReports and summaries are collections of plots. They can be generated automatically using the automated workflows, or they can be made manually after fine-tuning the analysis of track dynamics. |
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Make Summary Report |
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Make Comparison Plots |
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Plotting functionsThe autoimated workflows and the reports that can be generated with TrackMateR use several different plotting functions. |
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Make a plot of MSD data |
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Plot several (n) MSD curves |
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Make a plot of all tracks |
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Make a histogram of alpha values |
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Make a plot of cumulative distance over time |
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Make a histogram of D values |
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Make a histogram of displacements |
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Make a plot of displacement over time |
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Make a histogram of track durations |
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Make a histogram of fractal dimension |
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Make a histogram of intensities |
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Make a histogram of track density (number of neighbours) |
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Make a histogram of average speed |
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Make a histogram of the largest point-to-point distance in each track |
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Analysis of non-TrackMate dataTrackMateR can read and process csv files in a similar way to TrackMate XML outputs. These are typically “ground truth” data sets that have been generated synthetically, but could come from some other package. |
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Compare ground truth datasets |
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Read a ground truth csv file |